What is UV visible spectroscopy and what are its principles?

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Accepted Answer

UV visible refers to the measurement of light absorption and transmission in the ultraviolet and visible regions of the electromagnetic spectrum.

UV visible spectroscopy is a technique used to determine the concentration of a substance in a sample by measuring the amount of light absorbed or transmitted by the sample.
It is based on the principle that molecules absorb light at specific wavelengths, which can be used to identify and quantify the substance.
UV visible spectroscopy is commonly used in chemistry, biochemistry, and environmental science to analyze samples such as proteins, DNA, and pollutants.
Examples of UV visible applications include determining the concentration of a protein in a solution, monitoring the growth of bacteria in a culture, and measuring the amount of ozone in the atmosphere.

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