What methods do you use for counting microbial load?

Question

Accepted Answer

Microbial load can be counted using various methods including plate count, filtration, and most probable number (MPN) method.

Plate count method involves spreading a diluted sample on a solid agar medium and counting the colonies that grow after incubation.
Filtration method involves filtering a known volume of sample through a membrane filter and counting the colonies that grow on the filter after incubation.
MPN method involves diluting the sample and inoculating it into a series of tubes containing a liquid growth medium. The tubes are then observed for growth and the number of positive tubes is used to estimate the microbial load.
Other methods include direct microscopic count and turbidity measurement.
The choice of method depends on the type of sample, the microbial load expected, and the resources available.

Accepted Answer

including plate count, filtration, and most probable number (MPN) method.

Plate count method involves spreading a diluted sample on a solid agar medium and counting the colonies that grow after incubation.
Filtration method involves filtering a known volume of sample through a membrane filter and counting the colonies that grow on the filter after incubation.
MPN method involves diluting the sample and inoculating it into a series of tubes containing a liquid growth medium. The tubes are then observed for growth and the number of positive tubes is used to estimate the microbial load.
Other methods include direct microscopic count and turbidity measurement.
The choice of method depends on the type of sample, the microbial load expected, and the resources available.

Accepted Answer

Standard plate count

Accepted Answer

Standard plate count

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