What is peak purity in HPLC and what is its significance?

Question

Accepted Answer

Peak purity in HPLC refers to the homogeneity of a chromatographic peak and its significance is to ensure accurate identification and quantification of analytes.

Peak purity is determined by comparing the spectra of the peak at different points along its length.
A pure peak will have a consistent spectrum, while an impure peak will have variations in its spectrum.
Peak purity is important for accurate identification and quantification of analytes in a sample.
Impure peaks can lead to inaccurate results and misinterpretation of data.
Peak purity can be assessed using various methods such as spectral comparison, peak purity index, and purity angle.
For example, in pharmaceutical analysis, peak purity is critical for ensuring the quality and safety of drugs.
Peak purity is also important in environmental analysis to accurately measure the levels of pollutants in samples.

Accepted Answer

Peak purity in HPLC refers to the homogeneity of a chromatographic peak and its significance is to ensure accurate identification and quantification of analytes.

Accepted Answer

Spectral homogeneity across a peak is called peak purity. And it tell about that there is a co-illution or not in a peak.

What is peak purity in HPLC and what is its significance?

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