What are the types of detectors in HPLC and their applications?

Question

Accepted Answer

Detectors in HPLC identify and quantify compounds as they elute from the column, crucial for analysis.

UV-Visible Detector: Measures absorbance of UV/visible light; commonly used for organic compounds.
Fluorescence Detector: Detects compounds that fluoresce; highly sensitive for trace analysis.
Refractive Index Detector: Measures changes in refractive index; useful for non-UV absorbing compounds.
Conductivity Detector: Measures ionic compounds; often used in pharmaceutical and environmental analysis.
Mass Spectrometry (MS): Provides molecular weight and structure information; used for complex mixtures.

Accepted Answer

UV, VIS, and PDA Detectors.

Interview Questions from Popular Companies